American Journal of Ophthalmology
Volume 139, Issue 1 , Pages 168-178 , January 2005

Long-term histopathologic findings in human corneal wounds after refractive surgical procedures

  • Daniel G. Dawson, MD

      Affiliations

    • Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
  • ,
  • Henry F. Edelhauser, PhD

      Affiliations

    • Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
  • ,
  • Hans E. Grossniklaus, MD

      Affiliations

    • Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
    • Corresponding Author InformationInquiries to Hans E. Grossniklaus, MD, L.F. Montgomery Laboratory, BT 428, Emory Eye Center, 1365-B Clifton Road, N.E., Atlanta, Georgia 30322; fax (404) 778-2244

,Accepted 30 August 2004.

References 

  1. Trokel SL , Srinivasan R , Braren B . Excimer laser surgery of the cornea . Am J Ophthalmol . 1983;96:710–715
  2. Leaming DV . Practice styles and preferences of ASCRS member-2003 survey . J Cataract Refract Surg . 2004;30:892–900
  3. Anderson NJ , Edelhauser HF , Sharara N , et al.   Histologic and ultrastructural findings in human corneas after successful laser in situ keratomileusis . Arch Ophthalmol . 2002;120:288–293
  4. Kramer TR, Chuckpaiwong V, Dawson DG, et al. Pathologic findings in the cornea after successful LASIK surgery. Cornea. Forthcoming.
  5. Dawson DG, Kramer TR, Grossniklaus HE, et al. Histologic, ultrastructural, and immunofluorescent evaluation of human laser in situ keratomileusis corneal wounds. Arch Ophthalmol. Forthcoming.
  6. Dawson DG, Holley GP, Geroski DH, Waring GO, Grossniklaus HE, Edelhauser HF. Ex vivo confocal microscopy of human LASIK corneas with histologic and ultrastructural correlation. Ophthalmology. Submitted.
  7. Spirn MJ, Dawson DG, Rubinfeld RS, et al. Histopathology of post-LASIK corneal ectrasia with intrastromal corneal ring segments. Arch Ophthalmol. Forthcoming.
  8. Williams KK , Noe RL , Grossniklaus HE , Drews-Botsch C , Edelhauser HF . Correlation of histologic corneal endothelial cells counts with specular microscopic cell density . Arch Ophthalmol . 1992;110:1146–1149
  9. Maurice DM . The biology of wound healing in the corneal stroma . Cornea . 1987;6:162–168
  10. Assil K , Quantock AJ . Wound healing in response to keratorefractive surgery . Surv Ophthalmol . 1993;38:289–302
  11. Kitano S , Goldman JN . Cytologic and histochemical changes in corneal wound repair . Arch Ophthalmol . 1966;76:345–354
  12. Matsuda H , Smelser GK . Electron microscopy of corneal wound healing . Exp Eye Res . 1973;16:427–442
  13. Mohan RR , Hutheon AEK , Choi R , et al.   Apoptosis, necrosis, proliferation, and myofibroblast generation in the stroma following LASIK and PRK . Exp Eye Res . 2003;76:71–87
  14. Binder PS . Barraquer lecture (what we have learned about corneal wound healing from refractive surgery) . Refract Corneal Surg . 1989;5:98–120
  15. Melles GRJ , Binder PS , Anderson JA . Variation in healing throughout the depth of long-term, unsutured, corneal wounds in human autopsy specimens and monkeys . Arch Ophthalmol . 1994;112:100–108
  16. Melles GRJ , Binder PS , Moore MN , Anderson JA . Epithelial-stromal interactions in human keratotomy wound healing . Arch Ophthalmol . 1995;113:1124–1130
  17. Lang GK , Green WR , Maumenee AE . Clinicopathologic studies of keratoplasty eyes obtained post mortem . Am J Ophthalmol . 1986;101:28–40
  18. Melles GR , Binder PS . A comparison of wound healing in sutured and unsutured corneal wounds . Arch Ophthalmol . 1990;108:1460–1469
  19. Deg JK , Binder PS . Wound healing after astigmatic keratotomy in human eyes . Ophthalmol . 1987;94:1290–1298
  20. Flaxel JT , Swan KC . Limbal wound healing after cataract extraction . Arch Ophthalmol . 1969;81:653–659
  21. Vesaluoma M , Perez-Santonja J , Petroll WM , Linna T , Alio J , Tervo T . Corneal stromal changes induced by myopic LASIK . Invest Ophthalmol Vis Sci . 2000;41:369–376
  22. Mitooka K , Ramirez M , Maguire LJ , et al.   Keratocyte density of central human cornea after laser in situ keratomileusis . Am J Ophthalmol . 2002;133:307–314
  23. Erie JC , Patel SV , McLaren JW , et al.   Effect of myopic laser in situ keratomileusis on epithelial and stromal thickness . Ophthalmology . 2002;109:1447–1452
  24. Perez-Gomez I , Efron N . Change to corneal morphology after refractive surgery (myopic laser in situ keratomileusis) as viewed with a confocal microscope . Optomet Vis Sci . 2003;80:690–697
  25. Dillon EC , Eagle RC , Laibson PR . Compensatory epithelial hyperplasia in human corneal disease . Ophthalmic Surg . 1992;23:729–732
  26. Wilson SE , Liu JJ , Mohan RR . Stromal-epithelial interactions in the cornea . Prog Retinal Eye Res . 1999;18:293–309
  27. Moilanen JA , Vesaluoma MH , Muller LJ , Tervo TM . Long-term corneal morphology after PRK by in vivo confocal microscopy . Invest Ophthalmol Vis Sci . 2003;44:1064–1069
  28. Cintron C , Hassinger LC , Kublin CL , Cannon DJ . Biochemical and ultrastructural changes in collagen during corneal wound healing . J Ultrastruct Res . 1978;65:13–22
  29. Cintron C , Covington H , Kublin C . Morphogenesis of rabbit corneal stroma . Invest Ophthalmol Vis Sci . 1983;24:543–556
  30. Nakayasu K , Tanaka M , Konomi H , Hayashi T . Distribution of type I, II, III, IV, and V collagen in normal and keratoconus corneas . Ophthalmic Res . 1986;18:1–10
  31. Gregory JD , Damle SP , Covington HI , Cintron C . Developmental changes in proteoglycans of rabbit corneal stroma . Invest Ophthalmol Vis Sci . 1988;29:1413–1417
  32. Funderburgh JL , Chandler JW . Proteoglycans of rabbit corneas with nonperforating wounds . Invest Ophthalmol Vis Sci . 1989;30:435–442
  33. Cintron C , Hong BS , Covington HI , Macarak EJ . Heterogenity of collagens in rabbit cornea (type III collagen) . Invest Ophthalmol Vis Sci . 1988;29:767–775

 Supported in part by National Eye Institute grants EY-00933, P30-EY06360, and T32-EY07092 and by an unrestricted grant from Research to Prevent Blindness.

PII: S0002-9394(04)01091-8

doi: 10.1016/j.ajo.2004.08.078

American Journal of Ophthalmology
Volume 139, Issue 1 , Pages 168-178 , January 2005