Advertisement

A Pilot Study of Fourier-Domain Optical Coherence Tomography of Retinal Dystrophy Patients

  • Jennifer I. Lim
    Correspondence
    Inquiries to Jennifer I. Lim, 1855 W. Taylor Street, Mail Code 648, Chicago, IL 60612
    Affiliations
    Department of Ophthalmology, Eye and Ear Infirmary, University of Illinois, Chicago, Illinois

    Doheny Eye Institute, Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, California
    Search for articles by this author
  • Ou Tan
    Affiliations
    Doheny Eye Institute, Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, California
    Search for articles by this author
  • Amani A. Fawzi
    Affiliations
    Doheny Eye Institute, Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, California
    Search for articles by this author
  • J. Jill Hopkins
    Affiliations
    Doheny Eye Institute, Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, California

    Retina Vitreous Associates, Los Angeles, California
    Search for articles by this author
  • John H. Gil-Flamer
    Affiliations
    Doheny Eye Institute, Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, California
    Search for articles by this author
  • David Huang
    Affiliations
    Doheny Eye Institute, Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, California
    Search for articles by this author

      Purpose

      To characterize the macular anatomy of retinal dystrophy eyes using high-speed, high-resolution, Fourier-domain optical coherence tomography (FD-OCT).

      Design

      Case-control study.

      Methods

      Retinal dystrophy patients and normal age- and gender-matched controls underwent FD-OCT imaging using the RTVue (Optovue Inc, Fremont, California, USA). Vertical and horizontal 8-mm scans of 1024 lines/cross-section were obtained. Based on boundaries manually drawn on computer displays of OCT cross-sections, the thicknesses of the retina, inner retinal layer (IRL), and outer retinal layer (ORL) were averaged over both 5-mm (macular) and 1.5-mm (foveal) regions centered at the fovea. The IRL was the sum of nerve fiber layer (NFL), ganglion cell layer (GCL), and inner plexiform layer (IPL) thicknesses. Total retinal thickness (RT) was measured between the internal limiting membrane (ILM) and the retinal pigment epithelium. ORL thickness was calculated by subtracting IRL thickness from RT.

      Results

      Fourteen patients (three retinitis pigmentosa, two cone-rod degeneration, two Stargardt disease, and seven normal controls) underwent FD-OCT imaging. Mean foveal RT was 271.3 ± 23.3 μm for controls and 158.4 ± 47.1 μm for retinal dystrophy patients (P < .001). Mean macular RT was 292.8 ± 8.1 μm for controls and 199.1 ± 32.6 μm for retinal dystrophy patients (P < .001). Mean macular ORL was 182.9 ± 4.7 μm for controls and 101.3 ± 18.7 μm for retinal dystrophy patients (P < .001); mean macular IRL was 109.9 ± 6.4 μm for controls and 97.9 ± 20.7 μm for retinal dystrophy patients (P = .06).

      Conclusion

      Eyes with retinal dystrophy had a small (11%) decrease in macular IRL and severe (45%) decrease in macular ORL compared to normal controls.
      To read this article in full you will need to make a payment

      References

        • Huang D.
        • Swanson E.A.
        • Lin C.P.
        • et al.
        Optical coherence tomography.
        Science. 1991; 254: 1178-1181
        • Fujimoto J.G.
        • Huang D.
        • Hee M.R.
        • et al.
        Physical properties of optical coherence tomography.
        in: Schuman J.S. Pulido C.A. Fujimoto J.G. Optical Coherence Tomography of Ocular Diseases. 2nd ed. SLACK Inc, Thorofare, New Jersey2004: 677-688
        • Huang D.
        • Ou T.
        • Fujimoto J.
        • et al.
        Optical coherence tomography.
        in: Huang D. Kaiser P.K. Lowder C.Y. Traboulsi E.I. Retinal Imaging. Mosby Elsevier, Philadelphia, Pennsylvania2006: 47-65
        • Wojtkowski M.
        • Leitgeb R.
        • Kowalczyk A.
        • et al.
        In vivo human retinal imaging by Fourier-domain optical coherence tomography.
        J Biomed Opt. 2002; 7: 457-463
        • Choma M.A.
        • Sarunic M.V.
        • Yang C.
        • Izatt J.A.
        Sensitivity advantage of swept source and Fourier-domain optical coherence tomography.
        Opt Express. 2003; 11: 2183-2189
        • Leitgeb R.
        • Hitzenberger C.K.
        • Fercher A.F.
        Performance of fourier domain vs. time domain optical coherence tomography.
        Opt Express. 2003; 11: 889-894
        • Wojtkowski M.
        • Srinivasan V.
        • Fujimoto J.G.
        • et al.
        Three-dimensional retinal imaging with high-speed ultra-high-resolution optical coherence tomography.
        Ophthalmology. 2005; 112: 1734-1746
        • Witkin A.J.
        • Ko T.H.
        • Fujimoto J.G.
        • et al.
        Ultra-high resolution optical coherence tomography assessment of photoreceptors in retinitis pigmentosa and related diseases.
        Am J Ophthalmol. 2006; 142: 945-952
        • Drexler W.
        • Morgner U.
        • Ghanta R.K.
        • et al.
        Ultra-high-resolution ophthalmic optical coherence tomography.
        Nat Med. 2001; 7: 502-507
        • Drexler W.
        • Sattmann H.
        • Hermann B.
        • et al.
        Enhanced visualization of macular pathology with the use of ultra-high-resolution optical coherence tomography.
        Arch Ophthalmol. 2003; 121: 695-706
        • Srinivasan V.J.
        • Wojtkowski M.
        • Witkin A.J.
        • et al.
        High-definition and 3-dimensional imaging of macular pathologies with high-speed ultra-high-resolution optical coherence tomography.
        Ophthalmology. 2006; 113: 2054.e1-2054.e14
        • Ko T.H.
        • Fujimoto J.G.
        • Schuman J.S.
        • et al.
        Comparison of ultra-high- and standard-resolution optical coherence tomography for imaging macular pathology.
        Ophthalmology. 2004; 111: 2033-2043
        • Ergun E.
        • Hermann B.
        • Wirtitsch M.
        • et al.
        Assessment of central visual function in Stargardt disease/fundus flavimaculatus with ultrahigh-resolution optical coherence tomography.
        Invest Ophthalmol Vis Sci. 2005; 46: 310-316
        • Huber R.
        • Adler D.C.
        • Fujimoto J.G.
        Buffered Fourier-domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s.
        Opt Lett. 2006; 31: 2975-2977
        • Pagon R.A.
        Retinitis pigmentosa.
        Surv Ophthalmol. 1988; 33: 137-177
        • Milam A.H.
        • Li Z.Y.
        • Fariss R.N.
        Histopathology of the human retina in retinitis pigmentosa.
        Prog Retin Eye Res. 1998; 17: 175-205
        • Tucker G.S.
        • Jacobson S.G.
        Morphological findings in retinits pigmentosa with early diffuse rod dysfunction.
        Retina. 1988; 8: 30-41
        • Heckenlively J.R.
        Retinitis pigmentosa.
        in: J.B. Lippincott Co, Philadelphia, Pennsylvania1988: 55-60
        • The Advanced Glaucoma Intervention Study Investigators
        The Advanced Glaucoma Intervention Study (AGIS): 1.
        Control Clin Trials. 1994; 15: 299-325

      Biography

      Dr Jennifer I. Lim is a Professor of Ophthalmology and Director of the Retina Service at University of Illinois. Her research encompasses clinical trials, retinal imaging and translational research. Her leadership positions include Retina Subcommittee Chair of the AAO AMPC, VP of WIO and Editorial Boards. Dr Lim has received the AAO Achievement Award, ASRS Honor Award, teaching awards, and Best Doctors in America. She has over 150 articles, book chapters, and edited several books including Age-Related Macular Degeneration.

      Biography

      Dr David Huang is the Manager Chair in Corneal Laser Surgery, an Associate Professor of Ophthalmology, and a Biomedical Engineering at the University of Southern California (USC), and the Director of the Doheny Laser Vision Center (www.dohenylaser.com). Dr Huang earned his MD degree from Harvard Medical School and PhD from Massachusetts Institute of Technology. He did ophthalmology residency at USC and cornea fellowship at Emory University. Dr Huang is a co-inventor of optical coherence tomography.